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Design And Construction Of The Expression Vector A Pcr Assembling Of Hc Tm Fusion Genefig

This post categorized under Vector and posted on June 10th, 2019.
Gene Vector PCR: Design And Construction Of The Expression Vector A Pcr Assembling Of Hc Tm Fusion Genefig

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08.07.2010 Design and construction of the expression vector (A) PCR vectorembling of HC-TM fusion gene. VH HC variable domain CH HC constant domain TM PDGFR TM domain. Five fragments were generated through five sequential PCR amplifications. The sequences of the primers P1P9 were listed in Table 1. (B) Cloning procedure of pDGB-HC-TM. Expression of the inserted gene was driven by CMV Cited by 11Publish Year 2010Author Ye Zhou Zhen-Rui Chen Chang-Zheng Li Wei He Shuwen Liu Shibo Jiang Shibo Jiang Wen-Li Ma WanAutor Zhou YeIf a target protein without vector-derived amino acids is required the pTXB1 or the pTYB21 vector can be used. If a C-terminal fusion is required where the C-terminus of the target protein is fused to the intein then the pTXB1 vector should be used. If a N-terminal fusion is Home Protocols Cloning a PCR Fragment Into a pMAL Expression Vector (E8200) Cloning a PCR Fragment Into a pMAL Expression Vector (E8200) It is vectorumed that the PCR fragment is approximately 1 kb begins with a blunt end and has a SbfI overhang at the 3 end.

Summary. In this chapter protocols for the construction of expression vectors using In-Fusion PCR cloning are presented. The method enables vector and insert DNA sequences to be seamlessly joined in a ligation-independent reaction.Cited by 62Publish Year 2009Author Nick S. Berrow David Alderton Raymond J. OwensOrt OxfordAbstract. The expression of cloned genes in mammalian cells is a basic tool for understanding gene expression protein structure and function and biological regulatory mechanisms.Cited by 137Publish Year 2000Author Randal J. KaufmanOrt Ann ArborFusion proteins or chimeric (k-mir-ik) proteins (literally made of parts from different sources) are proteins created through the joining of two or more genes that originally coded for separate proteins.


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